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Engineering a non-native hydrogen production pathway into Escherichia coli via a cyanobacterial [NiFe] hydrogenase

Part of a wider set of hydrogen-related papers, this industrially funded work demonstrates transfer of a Nickel-Iron hydrogenase from a photosynthetic organism into the industrial workhorse Escherichia coli. This class of hydrogenase is less oxygen sensitive than many hydrogenases and offers potential for a substantial contribution to the hydrogen economy. We demonstrate this process adds parallel capability to E.coli, thus generating more hydrogen. This opens up the possibility of E. coli becoming a commercially efficient and effective economic hydrogen biofuels producer.