Output details

Tuning the cavity of cyclodextrins: altered sugar adaptors in protein pores

Cited in prestigious Acc. Chem. Res. and Chem. Soc. Rev., this paper describes a novel method of stochastic sensing. b-cyclodextrin (bCD) has been used as a molecular adapter within the wild-type and mutant a-hemolysin pores to study host-guest molecular recognition. To extend the sensitivity of stochastic sensing, we designed and synthesized two stereo-isomeric CDs containing a disulphide to tune the cavity and shape of the bCD. Applications of these mutant pores and synthetic adapters for the detection of both symmetric and asymmetric molecules have been achieved, which expands the breadth in sensitivity and diversity of sensing with protein pores.