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Interfacial study of cell adhesion to liquid crystals using widefield surface plasmon resonance microscopy

WSPR microscope was also found to be an effective tool for interrogating cell-soft surface interactions. The focal adhesions and location of the nucleus found by immunofluorescence staining matched WSPR images and revealed cell body mass could contribute to different topographic features at cell-surface interface depending on the absence and presence of a soft substrate such as a thin liquid crystal (LC) film. Cell growth on LC resulted in reduced cell adhesion area associated with remodelling of focal adhesions. Theoretical simulation suggested LCs could shift the excitation angle to higher order yet provide sufficient reflectivity for surface plasmon resonance imaging