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Rapid acidification and alkylation: redox analysis of the MHC class I pathway.

Rapid acidification and alkylation methods can be used to characterise the redox status of oxidoreductase enzymes which are increasingly useful in industrial biocatalysis. In particular they enable quantification of free cysteine residues within an enzyme domain or subunit. This fundamental study provided greater understanding of the usefulness of rapid acidification and alkylation methods for redox analysis of large, complex oligomeric transmembrane proteins. This has lead to more sophisticated analysis of enzymes like transketolase (see BiCE impact case study) in which cysteine residues play an important role in thermo-resistance of stability and activity that might limit its industrial application.