Output details

Thermodynamic parameters for salt-induced reversible protein precipitation from automated microscale experiments.

The characterisation of protein precipitation profiles in a high-throughput manner will have significant impact on the ability of engineers to establish rapidly chromatographic and protein refolding processes that avoid unwanted protein precipitation. It will also enable the design and rapid optimisation of protein precipitation processes when used for early stage protein purification. This paper describes the first application of salt-induced protein precipitation in microtitre plates from which thermodynamic parameters could be measured with high accuracy. Such parameters will enable future modelling of chromatographic separations and prediction of precipitation profiles (for separation by fractional precipitation) using protein structure information.