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15 - General Engineering

Brunel University London

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Output 211 of 258 in the submission

Article title

Scale-up of protein purifications using aqueous two-phase systems: Comparing multilayer toroidal coil chromatography with centrifugal partition chromatography

Type

D - Journal article

DOI

10.1016/j.chroma.2011.04.013

Title of journal

Journal of Chromatography A

Article number

Volume number

1218

Issue number

First page of article

5527

ISSN of journal

00219673

Year of publication

2011

URL

http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271409&_user=545641&_pii=S002196731100481X&_check=y&_origin=article&_zone=toolbar&_coverDate=12-Aug-2011&view=c&originContentFamily=serial&wchp=dGLzVBA-zSkWz&md5=d3aec64035359166fe10d8f13a5a3557&p

Number of additional authors

Additional information

The paper is the outcome of a follow-on grant (BB/FOF/206) from the BBSRC protein purification study (BB/C506364/1). A new multilayer toroidal coil centrifuge (MTCC) was developed to overcome the loss of stationary phase found when using a centrifugal partition chromatography (CPC). It was found that stage efficiency rose dramatically (up to 25%) as the centrifugal force field was increased. Furthermore there was no loss of stationary phase, with the MTCC having five times the throughput of the CPC with the same resolving power. The commercial potential of this new technology will be exploited by Dynamic Extractions (www.dynamicextractions.com).

Interdisciplinary

Cross-referral requested

Research group

None

Proposed double-weighted

Double-weighted statement

Reserve for a double-weighted output

Non-English

English abstract