Output details
12 - Aeronautical, Mechanical, Chemical and Manufacturing Engineering
University of Sheffield : B - Chemical and Biological engineering
Change of Carbon Source Causes Dramatic Effects in the Phospho-Proteome of the Archaeon Sulfolobus solfataricus
Using a mass spectrometry proteomics technique developed for another purpose we dramatically recast understanding of the ubiquity and role of protein phosphorylation in the Archaea. Using our approach, we expand the known landscape of protein phosphorylation in all Archaea by 8 fold. Furthermore, this approach revealed a strong preference for phospho-tyrosine of >50%, where <10% be expected from the literature. This, coupled with the dramatic impact of carbon-source changes on the phoisphoproteome, fundamentally changes the understanding of phosphorylation in the Archaea. This collaboration with two German labs has led to 2 follow-up papers and two major grant applications.
