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Phase system selection with fractional factorial design for purification of recombinant cyanovirin-N from a hydroponic culture medium using centrifugal partition chromatography

This paper arose from a 2 year project involving Brunel Institute for Bioengineering (BIB), St George’s Hospital University, London and East Malling Research Centre, Kent. The project was funded by NIH. It involved the insertion of a gene into the tobacco plant (St George’s) to create the protein Cyanovirin-N (CV-N), which has anti-HIV activity. The transgenic plant was then cultured (East Malling) and the CV-N purified (BIB). To achieve this, fractional factorial experiment design was used to determine the optimum liquid-liquid phase system to use and the success of this approach in purifying the protein resulted in the publication.