Output details
15 - General Engineering
Keele University
Article title
Single molecule detection of 5-hydroxymethylcytosine through chemical modification and nanopore analysis
Type
D - Journal article
Title of journal
Angew Chem Int Ed Engl
Article number
-
Volume number
52
Issue number
16
First page of article
4350
ISSN of journal
1521-3773
Year of publication
2013
URL
-
Number of additional authors
2
Additional information
In this paper, we reported a means for single-molecule detection of 5-hydroxymethylcytosine (hmC) in DNA, which may be associated with autoimmune diseases and cancer. hmC in DNA can be specifically modified by peptides or sugars in a single step. Modified hmC bases can be readily distinguished at the single-molecule level when they are driven into a protein nanopore. The purely chemical method is a simple and cheap method alternative to the enzymatic modification of hmC. This is a VIP and was chosen as frontispiece; highlighted in ChemBioChem (2013, 14: 1289) and Faculty 1000; news on Spectroscopy NOW and Chemistry Views.
Interdisciplinary
-
Cross-referral requested
-
Research group
None
Proposed double-weighted
No
Double-weighted statement
-
Reserve for a double-weighted output
No
Non-English
No
English abstract
-
