Output details
15 - General Engineering
King's College London
Hyperpolarized (13)C magnetic resonance detection of carboxypeptidase G2 activity
This paper is the first to identify a reporter molecule (difluorobenzoyl glutamic acid) for measuring the activity of a gene directed pro-drug activating enzyme (GDEPT) carboxypeptidase. GDEPT is an approach for suicide gene cancer and currently the subject of a clinical trial. There are currently no imaging techniques able to measure the efficacy of this therapy. The imaging methods developed here probe the activity of enzyme expression directly which underpins this therapy. We have extended the work to in vivo imaging [Smyth, NMR Biomed, 2011] and developed an MRI contrast technique for imaging this promising treatment [Yann, Mol. Imag. Biol, 2013].