Output details

Construction and Modelling of an Inducible Positive Feedback Loop Stably Integrated in a Mammalian Cell-Line

One aspect of synthetic biology is understanding design principles of natural biological systems through construction and modelling of simplified regulatory networks. Feedback and feed-forward loops are essential regulatory motifs found in such networks. Paper characterises an inducible transcriptional Positive Feedback Loop (PFL) by observing dynamic behaviour of clonal populations of Chinese hamster ovary cells using time-lapse microscopy. Two versions of synthetic network are compared, and resulting dynamics are linked to different topologies using non-linear dynamical models to reproduce experimental observations. Work provides tool to characterise behaviour of PFL motifs, which are often found in naturally-occurring pathways.