Output details
15 - General Engineering
University of Oxford
Article title
Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.
Type
D - Journal article
Title of journal
Proc Natl Acad Sci U S A
Article number
-
Volume number
109
Issue number
8
First page of article
2919
ISSN of journal
0027-8424
Year of publication
2012
URL
-
Number of additional authors
7
Additional information
A fundamental problem in microscopy is that microscopes only image well features lying in the focal plane. We have developed a technique that allows images to be obtained along arbitrary three-dimensional trajectories. The master patent on this (US8498048) has been licensed to Carl Zeiss (www.zeiss.com, details available). This paper describes a neuroscience application where a focussed beam of light is scanned at high speed, without aberration, along an axon. Fluorescence is used to study an action potential travelling along the axon. This led to an MRC grant (MR/K015877/1 £1.2M –jointly with the Department of Physiology, Anatomy and Genetics, Oxford).
Interdisciplinary
-
Cross-referral requested
-
Research group
C - Electrical and Electronic Engineering
Proposed double-weighted
No
Double-weighted statement
-
Reserve for a double-weighted output
No
Non-English
No
English abstract
-
