Output details

Fluorescence lifetime endoscopy using TCSPC for the measurement of FRET in live cells

This paper describes a new medical device that combines fibre endoscopy with time-correlated single-photon counting-based fluorescence lifetime measurements. For the first time, it enables protein-protein interactions to be imaged within living cells inside the body rather than in a laboratory microscope. It has high translational potential as it can accurately measure fluorescence lifetime in label-free applications in vivo, via the ports in existing clinical endoscopes. For example, it will allow minimally-invasive molecular imaging to query tumour status or drug effects. Fruwith generated all the biological models and with Festy conceived and built the equipment and analysed the data.