Output details

Folding-competent and Folding-defective Forms of Ricin A Chain Have Different Fates after Retrotranslocation from the Endoplasmic Reticulum

a) The author, Dr SC Allen, made a substantial contribution to this research in developing a number of temperature dependent ricin mutants, some of which are used in the publication (these data were part of an earlier publication not included in this REF cycle). In addition a yeast knockout library was screened by the author to identify which strains tolerated the so called temperature sensitive mutants. In so doing the author laid down the foundations for the experiments to be conducted, in that some of the strains tested initially were identified as being tolerant to the expression of the temperature sensitive ricin mutants, and these strains were included in the final data set. Some figures incorporating immunoprecipitation experiments were conducted by the author, where the figure depicts the use of RTA delta, and certain yeast growth assays.

b) The manuscript was written in conjunction with the other authors, and where necessary a critique of other sections was provided, by way of ensuring the important conceptual ideas developed initially were incorporated into the manuscript.