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Mathematical Modelling of Protein Precipitation Based on the Phase Equilibrium for an Antibody Fragment from E. coli Lysis

Precipitation is an important operation in biopharmaceutical purification yet the mechanism of protein precipitation in multi-component solutions is not well understood. Existing models lack fundamental understanding of the process. Here a new model is developed based on how the protein solubility changes during the process. The model structure is generic and robust and adequately reflects the non-linearity of protein precipitation kinetics. It thus provides new fundamental insights to protein precipitation in multi-component, complex protein solutions and can be applied to industrial processes to improve process design, model-based optimisation and process control.