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12 - Aeronautical, Mechanical, Chemical and Manufacturing Engineering
University College London
Microwell Engineering Characterization for Mammalian Cell Culture Process Development
Selection of high producing cell lines normally occurs in static cultures bearing little resemblance to ultimate bioreactor conditions. This work described the first successful suspension culture of mammalian cells in shaken microwells. It used combined modelling (CFD) and experimental approaches to rigorously characterise culture hydrodynamics and gas-liquid mass transfer. A subsequent EngD, using high productivity industrial cell lines, showed microwell cultures enabled more reliable selection of cell lines suited to large scale culture. Our industrial partner, MedImmune, has automated this approach and now routinely uses it for early stage cell culture process development (Dr Ray Field, FieldR@medimmune.com).
