Output details
15 - General Engineering
Manchester Metropolitan University
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Output 0 of 0 in the submission
Article title
Electrochemical product detection of an asymmetric convective polymerase chain reaction
Type
D - Journal article
Title of journal
Biosensors and Bioelectronics
Article number
-
Volume number
25
Issue number
2
First page of article
400
ISSN of journal
09565663
Year of publication
2009
URL
-
Number of additional authors
4
Additional information
We performed for the first time thermo-convective PCR with asymmetric primers leading to single-stranded products, We combined this with electrochemical detection after labelling the products with osmium tetroxide bipyridine. Thermo-convective PCR takes place near a heated platinum or gold microwire. Any PCR machine is not needed, rather, each volume element of the 50 microliter PCR solution experiences thermal cycling by mere thermo-convective movement through regions of different temperatures. This paves the way for extremely simplified and, yet, sensitive quantitative genosensors.
Interdisciplinary
Yes
Cross-referral requested
-
Research group
B - Bioengineering Group
Proposed double-weighted
No
Double-weighted statement
-
Reserve for a double-weighted output
No
Non-English
No
English abstract
-
