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Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities

The discovery of new bacteria and microalgae for CO2 fixation is hampered by the lack of methods to identify hitherto-unculturable microorganisms (accounting for 99% in natural environment). This work is the first to demonstrate that single cell Raman micro-spectroscopy and imaging technology can be applied to identify microorganisms for CO2 fixation and quantify the fixation rate of microbial communities in seawater. This label-free technology paves the way for the development of Raman activated cell sorting.