Output details
12 - Aeronautical, Mechanical, Chemical and Manufacturing Engineering
University of Sheffield : B - Chemical and Biological engineering
A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies
The result of a research project fully funded by Pfizer in the USA (KD). This work addressed a fundamental issue underpinning development of biopharmaceutical manufacturing systems - long-term stability of recombinant protein production by mammalian cell factories. Overturning the prevailing theory that recombinant gene silencing was primarily epigenetic, we showed that the major molecular mechanism of instability for Pfizer’s cell lines was recombinant gene deletion. This work has underpinned new Sheffield Industrial CASE studentships with Pfizer and UCB on gene vector stability and synthetic gene promoter design respectively.
