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12 - Aeronautical, Mechanical, Chemical and Manufacturing Engineering

University of Sheffield : B - Chemical and Biological engineering

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Article title

Cell line specific control of polyethylenimine-mediated transient transfection optimized with "Design of experiments" methodology

Type

D - Journal article

DOI

10.1002/btpr.715

Title of journal

Biotechnology Progress

Article number

Volume number

Issue number

First page of article

179

ISSN of journal

87567938

Year of publication

2011

URL

http://www.ncbi.nlm.nih.gov/pubmed/22002934

Number of additional authors

Additional information

The result of collaborations between Sheffield and MedImmune (OD, RF) and Sheffield and Pall (PLL), combining the work of two Industrial CASE postgraduates funded by the EPSRC (BCT) and the BBSRC (CRJS). This paper pioneered the use of “Design of Experiments” statistical modelling to optimise a rapid means of biopharmaceutical manufacturing based on transient gene expression, which is now used frequently by biomanufacturing companies. The core statistical modelling approach has been extended via a EU FP7 grant to develop a new high-throughput cellular analysis product that predicts cell bioprocess performance (patent application filed; WO1317387.8).

Interdisciplinary

Cross-referral requested

Research group

None

Proposed double-weighted

Double-weighted statement

Reserve for a double-weighted output

Non-English

English abstract